Journal: iScience

Article Title: ARL13B regulates juxtaposed cilia-cilia elongation in BBSome dependent manner in Caenorhabditis elegans

doi: 10.1016/j.isci.2025.111791

Figure Lengend Snippet:

Article Snippet: Carl Zeiss microscope , Carl Zeiss , Axio Vert.A1.

Techniques: Virus, Recombinant, Software, Microscopy, Fluorescence

Journal: Journal of Virology

Article Title: Next-Generation Porcine Intestinal Organoids: an Apical-Out Organoid Model for Swine Enteric Virus Infection and Immune Response Investigations

doi: 10.1128/JVI.01006-20

Figure Lengend Snippet: Development of porcine intestinal organoids. Crypts were isolated from the jejuna of 1- to 3-month-old pigs, cultured in Matrigel matrix with organoid growth medium, and monitored daily under a microscope to verify the formation diameter of the organoids. The developed organoids were subjected to freeze-thaw cycling, and then their daily growth was observed under a microscope. Images in the top panels are enlarged representative organoids. Images were obtained with a Zeiss Vert A1 microscope.

Article Snippet: Images were obtained with a Zeiss Vert A1 microscope.

Techniques: Isolation, Cell Culture, Microscopy

Journal: Journal of Virology

Article Title: Next-Generation Porcine Intestinal Organoids: an Apical-Out Organoid Model for Swine Enteric Virus Infection and Immune Response Investigations

doi: 10.1128/JVI.01006-20

Figure Lengend Snippet: TGEV infection in porcine 2D intestinal organoids. (A) Organoids were dissociated and seeded on a Matrigel-precoated 24-well plate, and 2D monolayer organoids formed after 3 days in culture. (B) Then, 2D organoids were inoculated with TGEV, and samples were collected at the indicated time points for viral load detection by RT-qPCR. The dashed line represents the limit of detection. (C) TGEV-infected or mock-infected organoids were fixed at 48 hpi for staining with TGEV N protein (green). DAPI was used for nuclear staining. Images were obtained with a ZEISS Vert A1 microscope. Scale bars, 50 μm.

Article Snippet: Images were obtained with a Zeiss Vert A1 microscope.

Techniques: Infection, Quantitative RT-PCR, Staining, Microscopy

Journal: iScience

Article Title: Chronic carbon disulfide exposure induces parkinsonian pathology via α-synuclein aggregation and necrosome complex interaction

doi: 10.1016/j.isci.2023.107787

Figure Lengend Snippet: CS 2 leads to synaptic injury (A–C) Frozen sections of rat brains containing SNpc were co-stained with anti-SYP antibodies and anti-TH antibodies and observed by fluorescence microscope (A). The yellow line represents the location of the line analysis (B), and the intensity of SYP staining was counted (C). (D and E) Transmission electron microscopy (TEM) was performed on fixed sections of rat brains containing SNpc (D). The average areas of dendrites (Den), axon terminals (At), and their mitochondria were counted (E). (C), (E) show means ± SEM, p value is comparison with control group by t test .

Article Snippet: After staining, slides were visualized and captured using a confocal microscope (ZEISS, Axio Vert.A1).

Techniques: Staining, Fluorescence, Microscopy, Transmission Assay, Electron Microscopy, Comparison, Control

Journal: iScience

Article Title: Chronic carbon disulfide exposure induces parkinsonian pathology via α-synuclein aggregation and necrosome complex interaction

doi: 10.1016/j.isci.2023.107787

Figure Lengend Snippet: CS 2 activation necroptosis signaling in dopaminergic neurons (A and B) The midbrain protein was extracted and immunoblotted with anti-RIP1, anti-p-RIP1, anti-RIP3, anti-p-RIP3, anti-MLKL, and anti- p -MLKL antibody (A), and the proteins level was quantified (B). (C–E) Frozen sections of rat brains containing SNpc were co-stained with anti- p -MLKL and anti-TH antibodies (C). The yellow line represents the location of the line analysis (D), and the Intensity of p -MLKL staining was counted (E). (F) Frozen sections of rat brains containing SNpc were stained with In Situ Cell Detection Kit and observed by fluorescence microscope. (G) Transmission electron microscopy (TEM) was performed on fixed sections of rat brains containing SNpc. (B) and (E) show means ± SEM, p value is comparison with control group by t test.

Article Snippet: After staining, slides were visualized and captured using a confocal microscope (ZEISS, Axio Vert.A1).

Techniques: Activation Assay, Staining, In Situ, Fluorescence, Microscopy, Transmission Assay, Electron Microscopy, Comparison, Control

Journal: iScience

Article Title: Chronic carbon disulfide exposure induces parkinsonian pathology via α-synuclein aggregation and necrosome complex interaction

doi: 10.1016/j.isci.2023.107787

Figure Lengend Snippet: CS 2 induced necroptotic signaling activating and cell loss was attenuated by GSK872 (A) SH-SY5Y cells was cultured and exposed to dose-sequence CS 2 or exposed to 10mM CS 2 which pre-intervention with 5μM GSK872. (B and C) Protein of SH-SY5Y cells that dose-sequence CS 2 exposed was extracted and immunoblotted with anti-MLKL, and anti- p -MLKL antibody (B), and the proteins level was quantified (C). (D and E) Protein of SH-SY5Y cells that GSK872 interferes was extracted and immunoblotted with anti-MLKL and anti- p -MLKL antibody (D), and the proteins level was quantified (E). (F and G) SH-SY5Y cells after GSK872 intervention were pictured using a light microscope (F) and counted (G). (C), (E), and (G) show means ± SEM, p value is comparison with control group by t test .

Article Snippet: After staining, slides were visualized and captured using a confocal microscope (ZEISS, Axio Vert.A1).

Techniques: Cell Culture, Sequencing, Light Microscopy, Comparison, Control

Journal: iScience

Article Title: Chronic carbon disulfide exposure induces parkinsonian pathology via α-synuclein aggregation and necrosome complex interaction

doi: 10.1016/j.isci.2023.107787

Figure Lengend Snippet: CS 2 -induced α-synuclein aggregation/phosphorylation and necroptotic signaling activating was attenuated by ELN484228 (A) SH-SY5Y cells was cultured and exposed to dose-sequence CS 2 or exposed to 10mM CS 2 which pre-intervention with 5μM ELN484228. (B and C) Protein of SH-SY5Y cells that dose-sequence CS 2 exposed was extracted and immunoblotted with anti-MLKL, and anti- p -MLKL antibody (B), and the proteins level was quantified (C). (D and E) Protein of SH-SY5Y cells that ELN484228 interferes was extracted and immunoblotted with anti-MLKL, and anti- p -MLKL antibody (D), and the proteins level was quantified (E). (F and G) SH-SY5Y cells after ELN484228 intervention were pictured using a light microscope (F) and counted (G). (C), (E), and (G) show means ± SEM, p value is comparison with control group by t test .

Article Snippet: After staining, slides were visualized and captured using a confocal microscope (ZEISS, Axio Vert.A1).

Techniques: Phospho-proteomics, Cell Culture, Sequencing, Light Microscopy, Comparison, Control